https://creativecommons.org/licenses/by/4.0/
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dc.contributor.authorRafiee, Mahmoud‐Reza-
dc.contributor.authorSigismondo, Gianluca-
dc.contributor.authorKalxdorf, Mathias-
dc.contributor.authorFörster, Laura-
dc.contributor.authorBrügger, Britta-
dc.contributor.authorBéthune, Julien-
dc.contributor.authorKrijgsveld, Jeroen-
dc.date.accessioned2022-01-28T09:19:37Z-
dc.date.available2022-01-28T09:19:37Z-
dc.date.issued2020-05-01-
dc.identifier.issn1744-4292en_US
dc.identifier.urihttp://hdl.handle.net/20.500.12738/12271-
dc.description.abstractStreptavidin-mediated enrichment is a powerful strategy to identify biotinylated biomolecules and their interaction partners; however, intense streptavidin-derived peptides impede protein identification by mass spectrometry. Here, we present an approach to chemically modify streptavidin, thus rendering it resistant to proteolysis by trypsin and LysC. This modification results in over 100-fold reduction of streptavidin contamination and in better coverage of proteins interacting with various biotinylated bait molecules (DNA, protein, and lipid) in an overall simplified workflow.en_US
dc.language.isoen_USen_US
dc.publisherEMBO Pressen_US
dc.relation.ispartofMolecular systems biologyen_US
dc.subjectchemical derivatizationen_US
dc.subjectChIP-SICAPen_US
dc.subjectcontaminationen_US
dc.subjectproteomicsen_US
dc.subjectstreptavidinen_US
dc.subject.ddc570: Biowissenschaften, Biologieen_US
dc.titleProtease‐resistant streptavidin for interaction proteomicsen_US
dc.typeArticleen_US
tuhh.container.issue5en_US
tuhh.container.volume16en_US
tuhh.oai.showtrueen_US
tuhh.publication.instituteUniversität Heidelberg. Biochemie-Zentrumen_US
tuhh.publication.instituteUniversität Heidelbergen_US
tuhh.publisher.doi10.15252/msb.20199370-
tuhh.type.opus(wissenschaftlicher) Artikel-
dc.rights.cchttps://creativecommons.org/licenses/by/4.0/en_US
dc.type.casraiJournal Article-
dc.type.diniarticle-
dc.type.driverarticle-
dc.type.statusinfo:eu-repo/semantics/publishedVersionen_US
dcterms.DCMITypeText-
tuhh.container.articlenumbere9370-
item.creatorGNDRafiee, Mahmoud‐Reza-
item.creatorGNDSigismondo, Gianluca-
item.creatorGNDKalxdorf, Mathias-
item.creatorGNDFörster, Laura-
item.creatorGNDBrügger, Britta-
item.creatorGNDBéthune, Julien-
item.creatorGNDKrijgsveld, Jeroen-
item.fulltextNo Fulltext-
item.creatorOrcidRafiee, Mahmoud‐Reza-
item.creatorOrcidSigismondo, Gianluca-
item.creatorOrcidKalxdorf, Mathias-
item.creatorOrcidFörster, Laura-
item.creatorOrcidBrügger, Britta-
item.creatorOrcidBéthune, Julien-
item.creatorOrcidKrijgsveld, Jeroen-
item.grantfulltextnone-
item.cerifentitytypePublications-
item.languageiso639-1en_US-
item.openairecristypehttp://purl.org/coar/resource_type/c_6501-
item.openairetypeArticle-
crisitem.author.deptDepartment Biotechnologie-
crisitem.author.parentorgFakultät Life Sciences-
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Diese Ressource wurde unter folgender Copyright-Bestimmung veröffentlicht: Lizenz von Creative Commons Creative Commons